Only 10 years ago, the Nobel Prize-winning work was presented monsterregularly craves short, spaced symmetrical repetitions, As a new and powerful tool for genome editing. Since then, many exciting applications for the CRISPR-Cas9 system have been developed and proposed, giving a glimpse of what’s to come next.
Progress has occurred at an unprecedented pace. The astonishing breadth of applications supports a claim the researchers made at the time of original publication: that CRISPR will have a revolutionary impact on the way we can use DNA science. These applications have the potential to greatly impact the treatment of health conditions, the drug discovery process and even global climate change efforts. Here are three noteworthy applications:
Prevent heart attacks
Since heart attacks are one of the leading causes of death in the entire population, finding innovative approaches to preventive and reactive measures is essential. Researchers at It has partnered with biotech companies to use genome sequencing to examine health outcomes for heart attacks in patients with familial hypercholesterolemia. High cholesterol is a significant risk factor for heart disease and individuals with familial hypercholesterolemia are particularly at risk. Using CRISPR, researchers target two different genes to mitigate heart disease risk –PCSK9 And the ANGPTL3. These genes are directly involved in the production of cholesterol in the body, which Verve researchers aim to stop. Interestingly, this only requires changing or removing a single letter from the genome.
Given that this is one of the first experiments to explore using CRISPR and DNA editing technology directly in humans, the long-term effects are still unknown – which is significant because when genome editing is used in medical applications, permanent changes are made to DNA. The rhAmpSeq CRISPR Analysis System from Integrated DNA Technologies, for research use only, is one example of a technology that contributes to knowledge of the unwanted off-target effects of this form of gene editing. newly found that this tool successfully assisted in the annotation of homology- and homology-related repair events associated with CRISPR.
Involve CRISPR in It is expected to accelerate the development of new treatments for life-threatening conditions. Continuing to show how this concept became a reality and how it holds the potential to rewrite the future of medicine as we know it.
Influence on disease progression
Disease development is one area where genetic modification has been shown to be beneficial. An example of this is how it was used with , a brain infection often found in Southeast Asian countries, caused by a virus transmitted by the bite of an infected mosquito. It is not transmitted from person to person and its prevalence has increased in recent years.
With no viable treatment options in place, researchers turned to CRISPR to find a solution to reduce or eliminate the presence of Japanese encephalitis in the general public. Recent studies up to 2020, published in BMC genomicsdescribe how CRISPR could assist in the genotyping of . Specifically, another study of It was suggested that the Cas12 (DNA-targeting) and Cas-13 (RNA-targeting) approaches could be used for both new diagnoses and even treatments for this class of viral diseases. By using these methods to target virus-carrying mosquitoes for gene editing, scientists will be able to create more effective vaccines and other preventative treatments.
Although it is difficult to predict the direction of the virus as it develops, CRISPR technology offers a possible option for influencing the development of Japanese encephalitis and other similar infections.
combating climate change
With climate change still a major concern for the world, scientists are seeking alternative ways to create a more efficient food production backbone. Genetically modifying plants using It is a well-validated procedure that scientists have used for years (primarily using Cas9 and Cas12a). Genome editing can also be used to modify genes to improve heat and drought tolerance in plants.
Cas12b is a newer CRISPR system that can target multiple genes in a single step during the gene editing process. Since it allows researchers to process more than one DNA sequence at a time, more precise experiments can be done to examine gene expression. The University of Maryland College of Agriculture and Natural Resources found that Related to transcriptional activation, multiplexing during gene editing, and ligation of transcriptional interference. The results of their projects contribute to the fact that CRISPR is a useful tool for altering genetic traits and suppressing or activating isolated genes, giving scientists an opportunity to use what their DNA already gives them.
In agriculture, both old and new CRISPR methods serve as a way for researchers to investigate various diseases and invasive pests. Currently, efforts to increase tolerance to abiotic stress, manage harmful diseases, improve crop yields and enhance nutrition are part of the focus for creating the potential of transgenic plants that can or other adverse natural events.
A look at the future of CRISPR technology
Given these examples of what CRISPR can help researchers achieve, it is clear that there is no limit to the possibilities of what gene-editing technologies can offer. Although a lot has already been achieved in the first decade since CRISPR was discovered as a genome-editing tool, the technology will continue to evolve and expand on what we already know. In the next decade, life science researchers can expect even more innovations using CRISPR technology and other gene-editing tools. The priority going forward will be to continue looking for ways to ensure that these tools can be used safely and effectively. As the technology behind genome editing develops, more applications will undoubtedly be discovered – leading to more discoveries that could positively change our world.
About the author:
Demaris Mills is President of Integrated DNA Technologies, a global provider of genomics solutions driving developments that inspire scientists to realize big dreams and pursue accomplishments. Mills has nearly 20 years of extensive global experience in the areas of life sciences, genomics and diagnostics, and a proven track record of delivering high-impact outcomes while fostering a highly inclusive, collaborative and engaging team environment.
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